Journal: New biotechnology

Article Title: Clinically compatible advances in blood-derived endothelial progenitor cell isolation and reprogramming for translational applications.

doi: 10.1016/j.nbt.2021.02.001

Figure Lengend Snippet: Fig. 1. EPC morphology and gene expression. (A) Appearance of a freshly isolated EPC colony at passage 0 on day 9 of culture. (B) Appearance of EPCs in culture at passage 2, (C–F) EPCs express the endothelial cell specific markers CD31 (C, red, DAPI blue) and CD144 (D, green, DAPI blue) and pluripotency associated markers NANOG (E, red) and REX-1 (F, red, DAPI blue). Images A-B at 50X magnification, images C, D, E, F at 100X magnification.

Article Snippet: Primary EPCs were stained using specific antibodies against CD31 (Cat. no. BBA7, R&D Systems, Abingdon, UK), CD144 (Cat. no. MAB9381, R&D Systems) and REX1 (Cat. no. AF3598, R&D Systems), or by using ULEX (Cat. no. RL-1062-2, Vector Laboratories) or DAPI (Cat. no. H-1200-10, Vector Laboratories) at the dilution recommended by the manufacturer.

Techniques: Gene Expression, Isolation

Journal: Clinical Science

Article Title: Relaxin improves multiple markers of wound healing and ameliorates the disturbed healing pattern of genetically diabetic mice

doi: 10.1042/cs20130105

Figure Lengend Snippet: Figure 7 VEGFR-2 and VE-cadherin staining (A, B) Representative immunohistochemical VEGFR-2 staining of skin wounds samples from diabetic (db + /db + ) mice treated with vehicle (6 μl of 0.9 % NaCl/mouse per day) or RLX (25 μg/mouse per day, subcutaneously) at day 12. Arrows indicate staining positivity. (C, D) Representative immunohistochemical VE-cadherin staining of skin wounds samples from diabetic (db + /db + ) mice treated with vehicle (6 μl of 0.9 % NaCl/mouse per day) or RLX (25 μg/mouse per day, subcutaneously) at day 12. Arrows indicate staining positivity. (C, F) Quantification of VEGFR-2- and VE-Cadherin-positive cells per field in normoglycaemic (db + / + m) and diabetic (db + /db + ) mice treated with vehicle (6 μl of 0.9 % NaCl/mouse per day) or with RLX (25 μg/mouse per day, subcutaneously) at day 12. Each bar represents the mean +−S.D. of six animals. *P < 0.05 compared with db + / + m + vehicle; §P < 0.01 compared with db + / + m + vehicle; #P < 0.01 compared with db + /db + + vehicle.

Article Snippet: Slides were incubated overnight with primary antibody to detect CD31, CD34, MMP-11, VE-cadherin, VEGFR-1 (all from Abcam) and VEGFR-2 (Cell Signaling) as described previously [20].

Techniques: Staining, Immunohistochemical staining